An application that translates raw thermal melt curve data into more easily assimilated knowledge is described. This program, called ‘Meltdown’, performs four main activities: control checks, curve normalization, outlier rejection, and melt temperature (T_m) estimation. The final output is a report that summarizes the results of a Differential Scanning Fluorimetry experiment. The goal of Meltdown is not to replace human analysis of the raw fluorescence data, but to provide a meaningful and comprehensive interpretation of the data to make this useful experimental technique accessible to naïve users, as well as providing a starting point for detailed analyses by more experienced users.