From branching microtubule nucleation to mitotic spindle assembly: role and mechanism — ASN Events

From branching microtubule nucleation to mitotic spindle assembly: role and mechanism (#2)

Sabine Petry 1
  1. Princeton University, Princeton, NJ, United States

The microtubule (MT) cytoskeleton is essential for giving cells their shape, positioning organelles, serving as tracks that move materials, generating force for movement, and making mitotic and meiotic spindles to segregate chromosomes during cell division. Each function relies on a specific MT architecture, e.g. long and stable MTs in an axon or short and dynamic MTs arranged in a spindle. The MT cytoskeleton originates from several MT nucleation sites, a process which, despite its central biological importance, is poorly understood. I am using the mitotic spindle in Xenopus egg extracts as a MT model structure to research how MT nucleation is locally activated to build the MT cytoskeleton. The presentation will cover a new mechanism, by which microtubules are nucleated within the mitotic spindle. This mechanism plays a vital role in the self-organization of MTs into a bipolar structure, which is essential to ensure that the segregation of genetic material into daughter cells is as reliable as possible.